Roger Rabbit's been framed for murder and he's had to go to the private eye who "took the pictures" in order for help. Something foul is going on in Hollywood and Toontown and your only hope to save Roger Rabbit is to find Marvin Acme's lost will and testament before Judge Doom and his Weasel henchmen take justice into their own hands.

You play this game as the Private Investigator, Eddie Valliant, while Roger Rabbit wanders around the scenery behind you. Your job is to search the city for clues, items and punchlines to jokes which will be your only defense should the Weasels catch up to poor Roger. Except for these Weasels (and birds flying overhead), Roger is more or less invincible as a toon. That means for most of the gameplay, he will be ignored. Should the Weasels appear in an area with you however (with an alarm sounding as they get close), Roger will go bug-eyed and try to run away. It is then up to Eddie to keep the Weasels away from Roger or engage in knock-knock jokes should they happen to catch him.

The bulk of the gameplay takes place on side-scrolling areas that represent city blocks, caves and room interiors. There is also a top-view world map where you navigate between areas on foot or via Benny the Cab.